Product Name:
EGFR-pT693
Product Number:
ab-pk121
Target Full Name: Receptor protein-tyrosine kinase Epidermal growth factor receptor Thr-693 phosphosite
Target Alias: Avian erythroblastic leukemia viral (v-erb-b) oncogene homologue; Cell growth inhibiting protein 40; cell proliferation-inducing protein 61; ERBB; ERBB1; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Product Type Specific: EGFR protein kinase phosphosite-specific polyclonal antibody
Antibody Code: PK121
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T693
Protein UniProt: P00533
Protein SigNET: EGFR
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Peptide with amino acid sequence surrounding the human EGFR Thr-693 phosphosite
Antibody Immunogen Sequence: PL(pT)PS
Antibody Immunogen Description: 5 amino acid phosphopeptide corresponding to the human EGFR T693 phosphosite.
Production Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol
Storage Conditions: -20°C
Storage Stability: > 1 year
Product Use: Western blotting
Antibody Dilution Recommended: 2 µg/mL
Antibody Potency: Very strong
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Zebra fish.
Antibody Positive Control: T693 phosphorylated EGFR
Antibody Specificity: Very high
Antibody Cross Reactivity: No strong cross-reactivities with other proteins.
Related Product 1: EGFRSubtide kinase substrate peptide (Cat. No.: PE-01BHE95)
Related Product 2: EGFR T678 phosphorylation antibody (Cat. No.: AB-PK120)
Related Product 3: EGFR Y869 phosphorylation antibody (Cat. No.: AB-PK602)
Related Product 4: EGFR Y998 phosphorylation antibody (Cat. No.: AB-PK603)
Related Product 5: EGFR Y1069 phosphorylation antibody (Cat. No.: AB-PK599)
Related Product 6: EGFR Y1110 phosphorylation antibody (Cat. No.: AB-PK600)
Related Product 7: EGFR Y1172 phosphorylation antibody (Cat. No.: AB-PK601)
Related Product 8: EGFR expression antibody (Cat. No.: AB-NK052-5)
Related Product 9: EGFR expression antibody (Cat. No.: AB-NK052-6)
Related Product 10: EGFR expression antibody (Cat. No.: AB-NK052-4)
Scientific Background: EGFR (ERBB1, Erbb1, HER1) is a protein-tyrosine kinase of the TK group and EGFR family. It features three main domains: an extracellular ligand-binding domain (ECD), a transmembrane domain, and an intracellular tyrosine kinase domain. EGFR regulates cell proliferation, division, motility, survival, and has other roles in tissue development. Although the encoded protein is 134 kDa based on its amono acid sequence, EGFR undergoes extensive post-translational modifications, including phosphorylation and glycosylation, which affects its measured mass on SDS-PAGE gels (typically 170-180 kDa. It is activated by binding epidermal growth factor (EGF) and transforming growth factor-alpha (TGFa), which induce dimerization and autophosphorylation. Phosphorylation of Tyr-270 stimulates interaction with EGFR. Phosphorylation of Tyr-869 increases phosphotransferase activity and induces interaction with COX2. Phosphorylation of Tyr-1016 increases phosphotransferase activity and induces interaction with GrbB2, PLCg1, PTPN11 (SHP2), RasGAP, and Vav2. Phosphorylation of Tyr-1069 increases phosphotransferase activity and induces interaction with Cbl. Phosphorylation of Y1092 induces interaction with PLCg1, PTPN6 (SHP1) and Ras-GAP. Phosphorylation of Y1110 induces interaction with Ras-GAP. Phosphorylation of Tyr-1172 increases phosphotransferase activity and induces interaction with Dok1, EGFR, RasGAP, PTPN11 (SHP2), and Vav2. Phosphorylation of Tyr-1197 increases phosphotransferase activity and induces interaction with Cbl, EGR, PLCg1, RasGAP, SH3KBP1, Shc1, and PTPN6 (SHP1). EGFR is inhibited by phosphorylation at Thr-678, Ser-695 and Ser-1026. Phosphorylation of Thr-693, Ser-695, Ser-1070, Ser-1071, and Ser-1190 contributes to receptor internalization. EGFR is found on the plasma membrane, endosomes, and sometimes in the nucleus. It is a known oncoprotein (OP). Cancer-related mutations in human tumours point to a gain of function of the protein kinase. Constitutive activation of EGFR kinase activity has been seen with the mutations of V689M, E1005R+D1006K. Overexpression of EGFR, particularly in non-small cell lung cancer (NSCLC), lead to uncontrolled cell division. The active form of the protein kinase normally promotes tumour cell proliferation. This description may include information annotated by UniProt and/or Google AI.