Product Name:
Chk2-pT68
Product Number:
ab-pk120
Target Full Name: Protein-serine/threonine kinase Chk2 Thr-68 phosphosite
Target Alias: Cds1; CHEK2; CHK2 checkpoint homologue (S. pombe); EC 2.7.11.1; RAD53
Product Type Specific: Phosphosite-specific polyclonal antibody for human Chk2 T68 phosphosite
Antibody Code: PK119
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T68
Protein UniProt: O96017
Protein SigNET: O96017
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Peptide with amino acid sequence surrounding the human Chk2 Thr-68 phosphosite (Val-Ser-pThr-Gln-Glu)
Antibody Immunogen Sequence: VSpTQE
Antibody Immunogen Description: 5 amino acid phosphopeptide corresponding to the human Chk2 T68 phosphosite
Production Method: 5 amino acid phosphopeptide corresponding to the human Chk2 T68 phosphosite
Antibody Modification: Phosphosite-specific polyclonal antibody for human Chk2 T68 phosphosite
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol
Storage Conditions: -20°C
Storage Stability: > 1 year
Product Use: Western blotting, Immunohistochemistry (IHC)
Antibody Dilution Recommended: 2 µg/mL
Antibody Potency: Very strong
Antibody Species Reactivity: Human
Antibody Positive Control: T68 phosphorylated Chk2
Antibody Specificity: High
Antibody Cross Reactivity: Ocassionally, strong cross-reactivities with ~46 and ~100 kDa proteins can be detected.
Related Product 1: Chk2-pT68 phosphosite-specific antibody (Cat. No.: AB-PK581)
Related Product 2: Chk2-pT383 phosphosite-specific antibody (Cat. No.: AB-PK580)
Related Product 3: Cdc25C (205-225), KinSub - Cdc25C phosphatase (V205-R225, human) peptide; Chktide peptide
Related Product 4: Chk2Selectide - Chk2 (CHEK2) protein kinase substrate peptide
Scientific Background: Chk2 is a CAMK protein kinase of the Rad53 family that acts as a cell cycle checkpoint regulator and putative tumour suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage such as DNA double-strand breaks. It is rapidly phosphorylated at Thr-68 by MLTK in response to replication blocks and DNA damage, and activated through autophosphorylation. Chk2 phosphorylates CDC25C at Ser-216 and inhibits this phosphatase (blocking entry into mitosis), p53 at Thr-18 and Ser-20 (inducing cell cycle arrest in G1), and BRCA1 (maintaining survival after DNA damage). Twelve splice variant isoforms have been described for human Chk2. High expression is observed in testis, spleen, colon and peripheral blood leukocytes. Loss of function mutants of ChK2 can cause Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype often found with p53 mutations as well. Familial mutations also associated with prostate and breast cancers, and mutations also occur in a variety of sporadic cancers and cell lines.
