Product Name:
PYKSD8
Product Number:
ab-pk119
Product Type Specific: Generic phosphospecific antibody
Antibody Code: PG005
Antibody Target Type: Generic phosphospecific
Antibody Phosphosite: Generic phospho
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PYKSD8 phosphopeptides that featured the sequence W(pY)RAPE
Antibody Immunogen Description: Protein-serine/threonine kinase catalytic subdomain VII region
Production Method: Protein-serine/threonine kinase catalytic subdomain VII region
Antibody Modification: Generic phosphospecific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Related Product 1: PYK generic phosphotyrosine-specific antibody (Cat. No.: AB-PG001)
Related Product 2: PYK-Ultra generic phosphotyrosine-specific antibody (Cat. No.: AB-PG001U)
Related Product 3: Phosphotyrosine pan-specific antibody (Cat. No.: AB-CN005-2)
Scientific Background: This antibody cross-reacts with the phosphorylated form of the tyrosine residue that appears to be an inhibitory site in most protein-serine.threonine kinases (but not protein-tyrosine kinases), which is highly conserved and located two amino acid residues on the N-terminal side of the conserved alanine residue in kinase subdomain VIII "APE" sequence. Mutation of this site with either phosphomimetic glutamate or aspartate residues, or with a phenylalanine residues leads to loss of protein-serine/threonine kinase activity for a wide range of protein kinases.
