Product Name:
Calcineurin A
Product Number:
ab-np049-1
Target Full Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Target Alias: Alpha isoform formerly PPP2B; Calcineurin A alpha; Calcineurin A1; CalcineurinA; Calmodulin dependent calcineurin A subunit alpha isoform; Calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA; CALNA 1; CAM PRP catalytic subunit; CCN 1; Protein phosphatase 2B catalytic subunitProtein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform; Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA; Protein phosphatase 3 catalytic subunit alpha isozyme
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP049-1
Antibody Target Type: Pan-specific
Protein UniProt: Q08209
Protein SigNET: Calcineurin A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after human Calcineurin A
Antibody Immunogen Sequence: AVCEFLQHNNLLSILRAHEA
Antibody Immunogen Description: Corresponds to amino acid residues A264-A283.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4; 50% glycerol, 0.09% azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:120), IHC (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~61 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rabbit | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SPC-175 was sufficient for detection of Calcineurin A in 20 µg of Heat shock Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Scientific Background: Calcineurin is a heterodimeric phosphatase protein, also known as calcium-dependent serine-threonine phosphatase. The structure consists of a catalytic subunit alpha, Calcineurin A (57-59 kDa) the active site and a Ca2+ binding unit, Calcineurin B (19-20 kDa) the regulatory subunit (1). Binding of Ca2+-calmodulin to the regulatory domain removes an autoinhibitory segment from the catalytic site, activating the enzyme. Calcineurin plays a key role in the T-cell response growth and differentiation mechanism, regulating the activation of the Nuclear factor of activated T-cells (NFATc) which are important in the expression of IL-2 genes. It is widely distributed in tissues but found in exceptionally high levels in the brain. Calcineurin has been the target of inhibitors, the novel and structural immune-suppressants antifungal drugs (2). Genetic studies in yeast and fungi established the molecular basis of the inhibition mechanism by cyclosporin A-cyclophilin and FK506-FKBP12 (3). Dysfunction of Calcineurin is linked to Alzheimer’s disease, cardiac hypertrophy, and various developmental defects. This description may include information annotated by UniProt and/or Google AI.