Product Name:
DUSP11-3
Product Number:
ab-np048-3
Target Full Name: Dual specificity protein phosphatase 11
Target Alias: DUS11; PIR1; RNA,RNP complex-1 interacting phosphatase
Product Type Specific: DUSP11 protein phosphatase pan-specific antibody
Antibody Code: NP048-3
Antibody Target Type: Pan-specific
Protein UniProt: O75319
Protein SigNET: DUSP11
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP11 sequence peptide Cat. No.: PE-01BAK95
Antibody Immunogen Sequence: CSHRRYSWNVKPNASR
Antibody Immunogen Description: Corresponds to amino acid residues S290 to R304.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-40 kDa.
Antibody Specificity: High
Related Product 1: DUSP11 (290-304) antibody blocking peptide (Cat. No.: PE-01BAK95)
Related Product 2: DUSP11 expression antibody (Cat. No.: AB-NP048-1)
Related Product 3: DUSP11 expression antibody (Cat. No.: AB-NP048-2)
Scientific Background: DUSP11 (Dual Specificity Phosphatase 11; PIR) is an atypical phosphatase localized in the nucleus that primarily acts primarily as an RNA phosphatase rather than a protein phosphatase. DUSP11 exhibits RNA 5'-triphosphatase and diphosphatase activities, and weak protein-tyrosine phosphatase activity. In addition, it displays in vitro phosphatase activity with ATP, ADP and O-methylfluorescein phosphate. As an atypical member of the dual-specificity protein phosphatase family, it contains the catalytic domain (HCXXXXXR) but lacks the N-terminal CH2 domain found in conventional MAPK-specific phosphatases. It is predominantly found in the nucleus, where it participates in mRNA metabolism. It binds to RNA, and its activity is critical for processing non-coding RNAs and restricting viruses like Hepatitis C. DUSP11 acts as a negative regulator of Toll-like receptor (TLR) signalling by dephosphorylating TGF-beta-activated kinase 1 (TAK1) in macrophages. DUSP11 is identified as a p53 target gene that can induce growth arrest. Conversely, it acts as an immunosuppressive and pro-neoplastic factor in lung adenocarcinoma (LUAD) by regulating RNA and activating RIG-I signaling, with high expression correlating with poor prognosis in some cancers. This description may include information annotated by UniProt and/or Google AI.