Product Name:
DUSP12-2
Product Number:
ab-np046-2
Target Full Name: Dual specificity protein phosphatase 12
Target Alias: Dual specificity tyrosine phosphatase YVH1
Product Type Specific: DUSP12 (YVH1) protein phosphatase pan-specific antibody
Antibody Code: NP046-2
Antibody Target Type: Pan-specific
Protein UniProt: Q9UNI6
Protein SigNET: DUSP12
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP12 sequence peptide Cat. No.: PE-01BAM99
Antibody Immunogen Sequence: CHREGSGPIAFAHKRM
Antibody Immunogen Description: Corresponds to amino acid residues H237 to M251.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 36-41 kDa.
Antibody Specificity: High
Related Product 1: DUSP12 (237-251) antibody blocking peptide (Cat. No.: PE-01BAM99)
Related Product 2: DUSP12 expression antibody (Cat. No.: AB-NP046-1)
Related Product 3: DUSP12 expression antibody (Cat. No.: AB-NP046-3)
Scientific Background: DUSP12 (Dual Specificity Phosphatase 12; hYVH1) is an atypical, evolutionary conserved phosphatase that acts as a survival factor protecting cells from stress and apoptosis. It is related to the DUSP family of dual specificity phosphatases that target both serine, threonine and tyrosine phosphorylated sites in proteins. It is also known as a Glucokinase-associated dual specificity phosphatase (GKAP), and glucokinase appears to be a substrate. DUSP12 contains a catalytic dual-specificity phosphatase domain and a unique C-terminal C2H2 zinc-finger domain, which is crucial for its function. It lacks the N-terminal CH2 domain found in typical Mitogen-Activated Protein Kinase Phosphatases (MKPs). As a Saccharomyces cerevisiae YVH1 orthologue, it regulates ribosome biogenesis, cell cycle progression (G2/M). DUSP12 can inactivate stress-activated MAP kinases such as JNK and p38, suppressing proinflammatory cytokines. It interacts with ZNF622, a protein involved in regulating cell cycle, and is implicated in ribosome maturation. Found primarily in the nucleus, it is ubiquitously expressed, with highest levels in the spleen, testis, ovary, and leukocytes. It promotes cell proliferation and motility, with high expression reported in various malignancies. It also has potential links to type 2 diabetes susceptibility. This description may include information annotated by UniProt and/or Google AI.