Product Name:
DUSP8-3
Product Number:
ab-np042-3
Target Full Name: Dual specificity protein phosphatase 8
Target Alias: Dual specificity protein phosphatase hVH-5
Product Type Specific: DUSP8 protein phosphatase pan-specific antibody
Antibody Code: NP042-3
Antibody Target Type: Pan-specific
Protein UniProt: Q13202
Protein SigNET: DUSP8
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP8 sequence peptide Cat. No.: PE-01BBI75
Antibody Immunogen Sequence: CGMSSDDAYRFVKDRR
Antibody Immunogen Description: Corresponds to amino acid residues G266 to R280.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Gorilla | Cow | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-66 kDa.
Antibody Specificity: Medium
Related Product 1: DUSP8 (266-280) antibody blocking peptide (Cat. No.: PE-01BBI75)
Related Product 2: DUSP8 expression antibody (Cat. No.: AB-NP042-1)
Scientific Background: DUSP8 (hVH-5) is a dual specificity phosphatase that exhibits activity towards both tyrosine-protein phosphate as well as with serine/threonine-protein phosphate. It features a Rhodanese domain (kinase interaction motif) and a tyrosine-protein phosphatase domain (active site). It acts as a negative regulator of MAPK signaling pathways (JNK, p38, and ERK) by dephosphorylating their phosphotyrosine and phosphoserine/threonine residues. It is highly expressed in the brain (and especially developing brain), heart, and skeletal muscle, where it functions as a cytoplasmic "gatekeeper" involved in cellular stress, proliferation, apoptosis, and metabolic regulation. It acts as a negative regulator of stress-induced ERK1/2 signalling in cardiac myocytes and regulates hypothalamic JNK signaling, influencing insulin sensitivity. Its expression is induced by nerve growth factor and insulin. It regulates metabolic effects induced by JNK activation in obesity. DUSP8 is linked to type 2 diabetes risk and is involved in colorectal and breast cancer progression, as well as cardiac ventricular remodeling. This description may include information annotated by UniProt and/or Google AI.