Product Name:
DUSP7-1
Product Number:
ab-np041-1
Target Full Name: Dual specificity protein phosphatase 7
Target Alias: Dual specificity protein phosphatase PYST2; DUS7; DUS7_HUMAN; PYST2
Product Type Specific: DUSP7 protein phosphatase pan-specific antibody
Antibody Code: NP041-1
Antibody Target Type: Pan-specific
Protein UniProt: Q16829
Protein SigNET: DUSP7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP7 sequence peptide Cat. No.: PE-01BBD90
Antibody Immunogen Sequence: MKNQLRGPPARAHMC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to M14; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-43 kDa.
Antibody Specificity: Medium
Related Product 1: DUSP7 (1-14) antibody blocking peptide (Cat. No.: PE-01BBD90)
Related Product 2: DUSP7 expression antibody (Cat. No.: AB-NP041-2)
Related Product 3: DUSP7 expression antibody (Cat. No.: AB-NP041-3)
Scientific Background: DUSP7 (PYST2) is a dual specificity phosphatase that dephosphorylates MAP kinases on phospho-tyrosine and phospho-threonine residues and negatively regulates their phosphotransferase activity in response to changes in the cellular environment. It features a C-terminal catalytic domain and an N-terminal regulatory domain. It particularly targets ERK1/2 for dephosphorylation at a "pTEpY" motif) and inactivation. PYST2-S is a shortened alternatively spliced, shorter variant of the PYST2 protein that lacks the catalytic domain, and may act as a negative regulator of the longer PYST2-L variant that contains the catalytic domain, although it is unclear whether this is by competing for transcription, translation or activation factors. DUSP7 is crucial for controlling the intensity and duration of MAPK signalling, and plays a significant role in embryonic development—specifically neural and cardiac mesoderm differentiation. DUSP7 shows high activity towards ERK2 and is involved in oocyte maturation, specifically aiding in nuclear envelope breakdown. DUSP7 is often implicated in cancer, with overexpression found in myeloid leukemia. In some studies, its downregulation contributes to cell injury (e.g., in septic acute kidney injury), while in other studies, its upregulation promotes drug resistance in breast cancer. This description may include information annotated by UniProt and/or Google AI.