Product Name:
DUSP6-1
Product Number:
ab-np040-1
Target Full Name: Dual specificity protein phosphatase 6
Target Alias: Dual specificity phosphatase 6; Dual specificity protein phosphatase PYST1; DUS6; MAP kinase phosphatase 3; Mitogen-activated protein kinase phosphatase 3; MKP3; MKP-3; PYST1
Product Type Specific: DUSP6 protein phosphatase pan-specific antibody
Antibody Code: NP040-1
Antibody Target Type: Pan-specific
Protein UniProt: Q16828
Protein SigNET: DUSP6
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP6 (MKP3) sequence peptide Cat. No.: PE-01BBA95
Antibody Immunogen Sequence: DTLRPVPFASEMAIC
Antibody Immunogen Description: Corresponds to amino acid residues D3 to I16; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-45 kDa.
Antibody Specificity: Medium
Related Product 1: DUSP6 (3-16) antibody blocking peptide (Cat. No.: PE-01BBA95)
Related Product 2: DUSP6 expression antibody (Cat. No.: AB-NP040-2)
Related Product 3: DUSP6 expression antibody (Cat. No.: AB-NP040-3)
Scientific Background: DUSP6 (MKP3) is a dual specificity phosphatase that dephosphorylates MAP kinase ERK1 (and ERK2) on both 'Thr-202' and 'Tyr-204', and inhibits its activity during mitosis following initial growth factor activation. DUSP6 plays complex roles in cellular differentiation, development, and as both a tumour suppressor or pro-oncogenic factor depending on cancer type. It features a C-terminal catalytic domain, an N-terminal Cdc25-like (CH2) domain, a kinase interaction motif (KIM) and a nuclear export signal (NES). Unlike many other dual-specificity phosphatases, DUSP6 is mainly localized in the cytoplasm. It is expressed at high levels in the heart and pancreas. Mutations in DUSP6 are associated with congenital hypogonadotropic hypogonadism, which can include symptoms like anosmia (loss of smell) and hearing loss. DUSP6 is implicated in Alzheimer's disease, where reduced levels are correlated with cognitive decline. It functions as a tumour suppressor in cancers like pancreatic and lung cancer, but can act in a pro-oncogenic manner in others, such as glioblastomas, thyroid carcinomas and breats cancer. This description may include information annotated by UniProt and/or Google AI.