Product Name:
DUSP5-1
Product Number:
ab-np039-1
Target Full Name: Dual specificity protein phosphatase 5
Target Alias: Dual specificity protein phosphatase hVH3; DUS5; DUS5_HUMAN; HVH3; VH3
Product Type Specific: DUSP5 (VH3) protein phosphatase pan-specific antibody
Antibody Code: NP039-1
Antibody Target Type: Pan-specific
Protein UniProt: Q16690
Protein SigNET: DUSP5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP5 (VH3) sequence peptide Cat. No.: PE-01BAX80
Antibody Immunogen Sequence: MKVTSLDGRQLRKMLC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to L15; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-42 kDa.
Antibody Specificity: Very high
Related Product 1: DUSP5 (1-15) antibody blocking peptide (Cat. No.: PE-01BAX80)
Related Product 2: DUSP5 expression antibody (Cat. No.: AB-NP039-2)
Related Product 3: DUSP5 expression antibody (Cat. No.: AB-NP039-3)
Scientific Background: DUSP5 (VH3) is a dual specificity nuclear phosphatase that dephosphorylates MAP kinase ERK1 (and ERK2) on both 'Thr-202' and 'Tyr-204', and inhibits its activity during mitosis following initial growth factor activation. It appears to be highly specific for ERK1/2, often described as an inducible nuclear anchor for ERK. DUSP5 contains a conserved kinase interaction motif (KIM) that is crucial for binding and inactivating ERK2, and it is stabilized when complexed with its substrate. It acts as a nuclear feedback inhibitor, controlling cell proliferation, differentiation, and survival. DUSP5 is implicated in various biological processes, including immune system development and vascular remodeling. It acts as an anti-inflammatory regulator by inhibiting NF-κB and suppressing cytokines like TNF-α and IL-6. It also plays a role in restricting cardiomyocyte hypertrophy. In vascular regulation, it is essential for normal vascular development, and its loss can lead to enhanced endothelial cell apoptosis. DUSP5 can induce granulocytic differentiation in certain cell types. While expressed in various tissues, the highest levels of DUSP5 are found in the brain and pancreas. It appears to play a role in BRAF-mutant thyroid cancer and other malignancies and is a target of p53. Its downregulation is associated with oncogenic progression, such as in ovarian cancer. This description may include information annotated by UniProt and/or Google AI.