Product Name:
CDC25A-1
Product Number:
ab-np030-4
Target Full Name: Cell division cycle 25A phosphatase
Target Alias: CDC25M3; M-phase inducer phosphatase 1; MPIP1
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP038-1
Antibody Target Type: Pan-specific
Protein UniProt: P30304
Protein SigNET: P30304
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDC25A sequence peptide Cat. No.: PE-01AZN99
Antibody Immunogen Sequence: MELGPEPPHRRRLLC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to L14; N-terminus
Production Method: Corresponds to amino acid residues M1 to L14; N-terminus
Antibody Modification: Protein phosphatase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with recombinant human CDC25A on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 58-66 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human CDC25B and CDC25C.
Related Product 1: CDC25A-1 blocking peptide
Related Product 2: CDC25A-2 pan-specific antibody (Cat. No.: AB-NP038-2)
Related Product 3: CDC25A-3 pan-specific antibody (Cat. No.: AB-NP038-3)
Scientific Background: CDC25A is a protein-tyrosine phosphatase that functions as a dosage-dependent inducer of mitotic progression. It directly dephosphorylates CDK1 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E.