Product Name:
DUSP3-1
Product Number:
ab-np030-2
Target Full Name: Dual specificity protein phosphatase 3
Target Alias: Dual specificity protein phosphatase VHR; DUS3; T-DSP11; VHR
Product Type Specific: DUSP3 (VHR) protein phosphatase pan-specific antibody
Antibody Code: NP030-2
Antibody Target Type: Pan-specific
Protein UniProt: P51452
Protein SigNET: DUSP3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP3 (VHR) sequence peptide Cat. No.: PE-01BAR99
Antibody Immunogen Sequence: ELSVQDLNDLLSDGC
Antibody Immunogen Description: Corresponds to amino acid residues E6 to G19; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 20-25 kDa.
Antibody Specificity: Very high
Related Product 1: DUSP3 (6-19) antibody blocking peptide (Cat. No.: PE-01BAR99)
Related Product 2: DUSP3 expression antibody (Cat. No.: AB-NP030-3)
Related Product 3: DUSP3 expression antibody (Cat. No.: AB-NP030-4)
Scientific Background: DUSP3 (VHR; Vaccinia H1-Related) exhibits phosphatase activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. It dephosphorylates and inactivates the MAP kinases ERK1, ERK2 and JNK as well as ErbB2, STAT5, FAK and nucleophosmin (NPM). It is an atypical PTP (Protein Tyrosine Phosphatase) member that lacks the N-terminal CH2 domain found in traditional MAP kinase phosphatases (MKPs), but still efficiently dephosphorylates ERK1/2 and JNK. It acts as a key regulator in cell proliferation, DNA damage repair, angiogenesis, and immune responses. It promotes genomic stability by regulating cell cycle checkpoints and repairing UV-induced lesions. DUSP3 is essential for proper endothelial cell tube formation (angiogenesis) and modulates M2-like macrophage polarization. Its activity is enhanced by tyrosine phosphorylation (e.g., via ZAP-70/Tyk2) or by interaction with kinases such as VRK3. DUSP3 deficiency leads to resistance against LPS-induced endotoxin shock. It is upregulated in cervical and prostate cancers but downregulated in certain breast and non-small cell lung cancers. This description may include information annotated by UniProt and/or Google AI.