Product Name:
PP5 (PPT)
Product Number:
ab-np021-4
Target Full Name: Serine/threonine protein phosphatase 5
Target Alias: PPP5; PPT; Protein phosphatase T
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP021-4
Antibody Target Type: Pan-specific
Protein UniProt: P53041
Protein SigNET: PP5 (PPT)
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Recombinant full length human PP5 protein
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium ~58 kDa protein band in A431 and HEK293 cell lysates as well as mouse brain lysate by Western blotting.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human.
Antibody Positive Control: 1 µg/ml of SMC-244 was sufficient for detection of PP5 in 15 µg of human A431 lysates by ECL analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: No other prominent cross-reactive proteins in A431, HEK293 and mouse brain lysates.
Related Product 1: PP5 (PPT) expression antibody (Cat. No.: AB-NP021-3)
Related Product 2: PPP5C S463 phosphorylation antibody (Cat. No.: AB-PP507)
Scientific Background: Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues, potentially playing a role in the regulation of cell growth and differentiation. PP5 (Protein Phosphatase 5 ; PPT) is a unique, widely expressed serine/threonine phosphatase that regulates critical cellular processes, including stress responses, DNA damage repair, and steroid hormone signaling. It is a 58 kDa protein featuring a tetratricopeptide repeat (TPR) domain that enables it to act as a co-chaperone with Hsp90. PP5 is predominantly a nuclear protein and interacts with CDC16 and CDC27. It is found in association with several proteins that influence intracellular signlaing-cascades intitiated by hormones (glucocorticoids) or cellular stress (hypoxia, oxidative stress and DNA Damage). It phosphorylates serine residues of skeletal muscle phorylatse and histone H1. It may also be involved in mitosis and RNA biogenesis regulation (1). It regulates MAPK-mediated growth, cell cycle checkpoint activation, and apoptosis inhibition, specifically via p53 and ATM/ATR pathways. PP5 is heavily involved in cancer progression (notably breast and renal cell carcinoma) by regulating Hsp90-dependent kinases and providing a survival advantage to tumour cells. This description may include information annotated by UniProt and/or Google AI.