Product Name:
DUSP2-2
Product Number:
ab-np008-3
Target Full Name: Dual specificity protein phosphatase 2
Target Alias: Dual specificity protein phosphatase PAC-1
Product Type Specific: DUSP2 (PAC-1) protein phosphatase pan-specific antibody
Antibody Code: NP008-3
Antibody Target Type: Pan-specific
Protein UniProt: Q05923
Protein SigNET: DUSP2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP2 (PAC1) sequence peptide Cat. No.: PE-01BAP90
Antibody Immunogen Sequence: CLLPDRALRTRLVRG
Antibody Immunogen Description: Corresponds to amino acid residues C69 to G83.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 31-34 kDa.
Antibody Specificity: Medium
Related Product 1: DUSP2 (69-83) antibody blocking peptide (Cat. No.: PE-01BAP90)
Related Product 2: DUSP2 expression antibody (Cat. No.: AB-NP008-2)
Related Product 3: DUSP2 expression antibody (Cat. No.: AB-NP008-4)
Scientific Background: DUSP2 (PAC1) is a dual specificity phosphatase that dephosphorylates and inactivated MAP kinases (ERK, p38, JNK). For example, it dephosphorylates ERK2 on both 'Thr-183' and 'Tyr-185', and inhibits its activity during mitotic cell cycle progression following initial growth factor activation. It limits inflammation by regulating STAT3 signalling and TH17 cell differentiation. It is primarily localized to the nucleus of cells in hematopoietic tissues, where it controls immune responses. Its expression can be induced by p53 to promote apoptosis. It acts as a tumour suppressor, and its reduced expression is linked to poor prognosis in various cancers (e.g., cervical, colon). DUSP2 deficiency can lead to prolonged MAPK signalling, which can promote cancer stem cell accumulation and chemoresistance, and decreased immune-mediated inflammation. This description may include information annotated by UniProt and/or Google AI.