Product Name:
DUSP4-3
Product Number:
ab-np007-4
Target Full Name: Dual specificity protein phosphatase 4
Target Alias: Dual specificity protein phosphatase hVH2; DUS4; DUSP4; MAP kinase phosphatase-2; Mitogen-activated protein kinase phosphatase-2; MKP2; VH2; VH2/TYP1
Product Type Specific: MKP2 (DUSP4; VH2) protein phosphatase pan-specific antibody
Antibody Code: NP007-4
Antibody Target Type: Pan-specific
Protein UniProt: Q13115
Protein SigNET: DUSP4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP4 (MKP2) sequence peptide Cat. No.: PE-01BAW85
Antibody Immunogen Sequence: CSWFMEAIEYIDAVKD
Antibody Immunogen Description: Corresponds to amino acid residues S257 to D271.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macque | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-40 kDa.
Antibody Specificity: High
Related Product 1: DUSP4 (257-271) antibody blocking peptide (Cat. No.: PE-01BAW85)
Related Product 2: DUSP4 expression antibody (Cat. No.: AB-NP007-2)
Related Product 3: DUSP4 expression antibody (Cat. No.: AB-NP007-3)
Scientific Background: DUSP4 (Dual Specificity Phosphatase 4; MKP2; VH2) is a nuclear protein found in the nucleoplasm that acts as a negative regulator of mitogen-activated protein (MAP) kinases, including ERK1, ERK2, JNK, and to a lesser extent p38 isoforms. It dephosphorylates both phosphoserine/threonine and phosphotyrosine residues, playing a crucial role in regulating cellular proliferation, differentiation, and stress responses. For example, DUSP4 dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185', and inhibits its activity during mitosis following initial growth factor activation. It features an N-terminal kinase-interacting motif (KIM) and a C-terminal catalytic phosphatase domain. Its expression is often induced by growth factors or environmental stress. DUSP4 is essential for neuron development, with expression changes linked to Alzheimer's disease and depression. It also plays a role in regulating megakaryocyte differentiation and is associated with cancer progression. This description may include information annotated by UniProt and/or Google AI.