Product Name:
DUSP1-1 (MKP-1)
Product Number:
ab-np006-4
Target Full Name: MAP kinase phosphatase 1 (CL100, VH1)
Target Alias: 3CH134; CL100; DUS1; DUSP1; ERP; HVH1; PTPN10; PTPN16
Product Type Specific: MKP1 protein phosphatase pan-specific antibody
Antibody Code: NP006-4
Antibody Target Type: Pan-specific
Protein UniProt: P28562
Protein SigNET: DUSP1 (MKP1)
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP1 (MKP1) sequence peptide Cat. No.: PE-01ASZ99
Antibody Immunogen Sequence: MVMEVGTLDAGGLRC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to R14; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 4 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macque | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 39-44 kDa.
Antibody Specificity: Medium
Related Product 1: DUSP1 (1-14) antibody blocking peptide (Cat. No.: PE-01ASZ99)
Related Product 2: DUSP1 (MKP1) expression antibody (Cat. No.: AB-NP006-2)
Related Product 3: DUSP1 (MKP1) expression antibody (Cat. No.: AB-NP006-2P)
Related Product 4: DUSP1 (MKP1) expression antibody (Cat. No.: AB-NP006-3)
Scientific Background: DUSP1 (MKP1) is a 39 kDa dual specificity phosphatase that dephosphorylates and inactivated MAP kinases (ERK, p38, JNK). For example, it dephosphorylates ERK2 on both 'Thr-183' and 'Tyr-185', and inhibits its activity during meiotic cell cycle progression. It acts as a key regulator of stress response, inflammation, and cell proliferation. USP1 is strongly induced by cellular stressors such as heat shock, oxidative stress, and growth factors, and produced as an early response protein. It features a catalytic domain (PTP_DSP_cys) and a Rhodanese Homology Domain. DUSP1 is highly expressed in the lung, liver, placenta, and pancreas, with lower levels in the heart, brain, and kidney. DUSP1 deficiency has been linked to increased sensitivity to apoptosis and inflammatory issues. DUSP1 is implicated in cancer progression and chemoresistance (to agents like cisplatin), functioning as both a tumour suppressor or promoter depending on the cancer type. Elevated levels of DUSP1 are associated with various cancers (melanoma, prostate, pancreatic). It plays a protective role against chronic inflammation and can be induced by corticosteroids. This description may include information annotated by UniProt and/or Google AI.