Product Name:
CDC25C-2
Product Number:
ab-np003-2
Target Full Name: Cell division cycle 25C phosphatase
Target Alias: CDC25; CDC25M1; Cell division cycle 25 C; M-phase inducer phosphatase 3; MPI1B; MPIP3
Product Type Specific: CDC25C protein phosphatase pan-specific antibody
Antibody Code: NP003-2
Antibody Target Type: Pan-specific
Protein UniProt: P30307
Protein SigNET: CDC25C
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDC25C sequence peptide Cat. No.: PE-01AZR99
Antibody Immunogen Sequence: EDSNQGHLIGDFSKVC
Antibody Immunogen Description: Corresponds to amino acid residues E275 to C290.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human CDC25C on protein dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macque | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-65 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Medium immunoreactivity on protein dot blots with recombinant human CDC25A, very weak immunoreactivity with recombinant human CDC25B.
Related Product 1: CDC25C (275-290) antibody blocking peptide (Cat. No.: PE-01AZR99)
Related Product 2: CDC25B expression antibody (Cat. No.: AB-NP002-2P)
Related Product 3: CDC25B expression antibody (Cat. No.: AB-NP002-3)
Related Product 4: CDC25B expression antibody (Cat. No.: AB-NP002-4)
Related Product 5: CDC25C expression antibody (Cat. No.: AB-NP003-3)
Related Product 6: CDC25A expression antibody (Cat. No.: AB-NP038-1)
Related Product 7: CDC25A expression antibody (Cat. No.: AB-NP038-2)
Related Product 8: CDC25A expression antibody (Cat. No.: AB-NP038-3)
Scientific Background: CDC25C (Cell Division Cycle 25C) is a protein-tyrosine phosphatase that functions as a dosage-dependent inducer of mitotic progression. When phosphorylated, it is highly effective in activating G2 cells into prophase. It directly dephosphorylates CDK1 and CDK2 (at Thr-14 and Tyr-15, which are phosphorylated by checkpoint kinases like CHK1 and Wee1 kinase) and activates their kinase activities. It also acts as a DNA damage checkpoint enzyme to ensures proper DNA repair before mitosis. Its phosphatase activity is heavily controlled through phosphorylation and dephosphorylation, specifically through kinases like PLK1/Aurora A and check-point kinases ATR/CHK1. While active in the nucleus during mitosis, it is also found in the cytoplasm and associated with centrosomes. High expression of CDC25C is often observed in poorly differentiated tumors and is linked to aggressive tumor features and poor prognosis in certain cancers. This description may include information annotated by UniProt and/or Google AI.