Product Name:
CDC25C-2
Product Number:
ab-np002-4
Target Full Name: Cell division cycle 25C phosphatase
Target Alias: CDC25; CDC25M1; Cell division cycle 25 C; M-phase inducer phosphatase 3; MPI1B; MPIP3
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP003-2
Antibody Target Type: Pan-specific
Protein UniProt: P30307
Protein SigNET: P30307
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDC25C sequence peptide Cat. No.: PE-01AZR99
Antibody Immunogen Sequence: EDSNQGHLIGDFSKVC
Antibody Immunogen Description: Corresponds to amino acid residues E275 to C290
Production Method: Corresponds to amino acid residues E275 to C290
Antibody Modification: Protein phosphatase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human CDC25C on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-65 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Medium immunoreactivity on protein dot blots with recombinant human CDC25A, very weak immunoreactivity with recombinant human CDC25B.
Related Product 1: CDC25C-2 blocking peptide
Related Product 2: CDC25C-3 pan-specific antibody (Cat. No.: AB-NP003-3)
Scientific Background: CDC25C is a protein-tyrosine phosphatase that functions as a dosage-dependent inducer of mitotic progression. When phosphorylated, it is highly effective in activating G2 cells into prophase. It directly dephosphorylates CDK1 and activates its kinase activity.
