Product Name:
CDC25B-1
Product Number:
ab-np002-3
Target Full Name: Cell division cycle 25B phosphatase
Target Alias: CDC25HU2; CDC25M2; Cell division cycle 25 B; M-phase inducer phosphatase 2; MPIP2
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP002-3
Antibody Target Type: Pan-specific
Protein UniProt: P30305
Protein SigNET: P30305
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDC25B sequence peptide Cat. No.: PE-01ASW95
Antibody Immunogen Sequence: MEVPQPEPAPGSALC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to L14; N-terminus
Production Method: Corresponds to amino acid residues M1 to L14; N-terminus
Antibody Modification: Protein phosphatase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with recombinant human CDC25B on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 62-68 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: Very weak immunoreactivity on protein dot blots with recombinant human CDC25C, but no immunoreactivity with CDC25A.
Related Product 1: CDC25B-1 blocking peptide
Related Product 2: CDC25B pan-specific antibody (Cat. No.: AB-NP002-2P)
Related Product 3: CDC25B-3 pan-specific antibody (Cat. No.: AB-NP002-2)
Scientific Background: CDC25B is a protein-tyrosine phosphatase that functions as a dosage-dependent inducer of mitotic progression. It is required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. It directly dephosphorylates CDK1 and stimulates its kinase activity.
