Product Name:
PCYT1B-PNT
Product Number:
ab-nn605-1
Target Full Name: CTP: phosphocholine cytidylyltransferase isoform B
Target Alias: CCT B; CCT-beta; CCTB; Choline-phosphate cytidylyltransferase B; CT B; CTB; CTP:phosphocholine cytidylyltransferase B; PCY1B; PCYT1B; phosphate cytidylyltransferase 1, choline, beta; Phosphorylcholine transferase B
Product Type Specific: PCYT1B pan-specific antibody
Antibody Code: NN605-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y5K3
Protein SigNET: PCYT1B
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human PCYT1B
Antibody Immunogen Sequence: CQAPHEKLTIAQARLGTP
Antibody Immunogen Description: Corresponds to amino acid residues C55 to P72.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.55 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Chicken.
Related Product 1: PCYT1A expression antibody (Cat. No.: AB-NN606-1)
Related Product 2: PCYT1A T342+S343 phosphorylation antibody (Cat. No.: AB-PN547)
Related Product 3: PCYT1A Y359+S362 phosphorylation antibody (Cat. No.: AB-PN548)
Related Product 4: PCYT1A S329+S331 phosphorylation antibody (Cat. No.: AB-PN561)
Related Product 5: PCYT1B S315+S319 phosphorylation antibody (Cat. No.: AB-PN546)
Scientific Background: PCYT1B (Phosphate cytidylyltransferase 1B, choline) is the rate limiting enzyme in the biosynthetic pathway for phosphatidylcholine. It catalyzes the reaction: CTP + phosphocholine = diphosphate + CDP-choline. It is an ambiquitous enzyme that resides in the cytoplasm of cells in an inactive form. Diacylglycerol, acidic phospholipids and fatty acids drive the binding of PCYT1B to the endoplasmic reticulum and stimulation of its catalytic activity. It is critical for membrane synthesis and lipid metabolism, with high expression in the brain, placenta, ocary, testis, and fetus. It belongs to the cytidylyltransferase family and possesses a catalytic core and helical domain nearly identical to PCYT1A, but lacks the specific nuclear localization signal found in PCYT1A. Multiple alternative splicing variants exist. The protein can be expressed in 35-kD and 42-kD forms, with 42-kD potentially being a post-translationally modified version. It is often found in the cytoplasm and associates with the endoplasmic reticulum. It plays a role in phosphatidylcholine compositional homeostasis, and has been associated with studies on neonatal respiratory distress syndrome and preeclampsia. This description may include information annotated by UniProt and/or Google AI.