Product Name:
NRP1[293-309]
Product Number:
ab-nn604-1
Target Full Name: Neuropilin-1
Target Alias: CD304; Neuropilin 1; Neuropilin-1; NRP; Transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R
Product Type Specific: NRP1 pan-specific antibody
Antibody Code: NN604-1
Antibody Target Type: Pan-specific
Protein UniProt: O14786
Protein SigNET: NRP1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human NRP1
Antibody Immunogen Sequence: CASSQYSTNWSAERSRLN
Antibody Immunogen Description: Corresponds to amino acid residues A293 to N309.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.3 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Related Product 1: NRP1 expression antibody (Cat. No.: AB-NN604-2)
Scientific Background: NRP1 (Neuropilin-1) is a 120-130 kDa transmembrane glycoprotein acting as a multifunctional co-receptor for various ligands, including VEGF and class 3 semaphorins. It plays key roles in axon guidance, angiogenesis, and tumour progression by modulating signaling for VEGFRs and Plexins. It features extracellular CUB (a1/a2), FV/FVIII (b1/b2), and MAM (c) domains, followed by a transmembrane region and a short cytoplasmic tail. It binds ligands via the a and b domains. The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Although it lacks intrinsic kinase activity, it enhances signaling of other receptors like VEGFR2 (for angiogenesis) and Plexins (for nerve guidance). Coexpression of NRP1 with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. It may also bind as various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. NRP1 is overexpressed in many cancers, promoting angiogenesis and immune evasion, and also acts as a host receptor for SARS-CoV-2 virus entry leading to COVID-19. This description may include information annotated by UniProt and/or Google AI.