Product Name:
NF-H-CT
Product Number:
ab-nn583-1
Target Full Name: Neurofilament heavy polypeptide
Target Alias: 200 kDa neurofilament protein; Neurofilament triplet H protein; Neurofilament, heavy polypeptide; NF-H
Product Type Specific: NF-H (NEFH) pan-specific antibody
Antibody Code: NN583-1
Antibody Target Type: Pan-specific
Protein UniProt: P12036
Protein SigNET: NF-H
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human NF-H
Antibody Immunogen Sequence: CSKPPEKATEDKAAKGK-cooh
Antibody Immunogen Description: Corresponds to amino acid residues S1005 to K1020.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.85 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Mouse.
Related Product 1: NF-L expression antibody (Cat. No.: AB-NN582-1)
Related Product 2: NF-M Y320 phosphorylation antibody (Cat. No.: AB-PN894)
Related Product 3: NF-M S620 phosphorylation antibody (Cat. No.: AB-PN893)
Related Product 4: NF-H S532 phosphorylation antibody (Cat. No.: AB-PN970)
Related Product 5: N-FH S606 phosphorylation antibody (Cat. No.: AB-PN971)
Related Product 6: N-FH S648 phosphorylation antibody (Cat. No.: AB-PN972)
Scientific Background: NF-H (NEFH) is a cytoskeletal protein that belongs to the intermediate filament family, and forms structural components of neuronal axons. Neurofilaments usually contain three intermediate filament proteins that are involved in the maintenance of neuronal caliber. NF-H is the largest of the three neurofilament subunits (NF-L, NF-M, NF-H), featuring a unique C-terminal tail that projects from the filament core to form cross-bridges with other neurofilaments and microtubules. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. NF-H may additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. NF-H works with NF-M to form cross-bridges that determine the caliber of myelinated axons, thereby influencing the speed of nerve impulse transmission.The C-terminus of NF-H contains over 40 Lys-Ser-Pro (KSP) peptide repeats, which are heavily phosphorylated in mature, axonal neurofilaments. This phosphorylation is critical for maintaining axonal structure and controlling axon caliber. While the actual molecular weight is 110 kDa, the protein migrates anomalously at 180-220 kDa on SDS-PAGE due to its high charge and phosphorylation. It is primarily located in axons, provides structural stability and regulates neurofilament spacing. Non-phosphorylated forms are found in dendrites and perikarya during development. NEFH gene variants are associated with a rare, risk-modifying, or causal role in some cases of sporadic amyotrophic lateral sclerosis (ALS). Mutations in the C-terminal KSP-repeat domain of NEFH, which are critical for neurofilament structural integrity, are believed to contribute to motor neuron neurofilamentous swellings, a key hallmark of ALS. This description may include information annotated by UniProt and/or Google AI.