Product Name:
NF-L-CT
Product Number:
ab-nn582-1
Target Full Name: Neurofilament light polypeptide
Target Alias: NEFL; Neurofilament, light polypeptide; NF68; NF-L
Product Type Specific: NF-L (NEFL) pan-specific antibody
Antibody Code: NN582-1
Antibody Target Type: Pan-specific
Protein UniProt: P07196
Protein SigNET: NF-L
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human NF-L
Antibody Immunogen Sequence: CKKVEGAGEEQAAKKKD-cooh
Antibody Immunogen Description: Corresponds to amino acid residues K528 to D543. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse.
Related Product 1: NF-H expression antibody (Cat. No.: AB-NN583-1)
Related Product 2: NF-M Y320 phosphorylation antibody (Cat. No.: AB-PN894)
Related Product 3: NF-M S620 phosphorylation antibody (Cat. No.: AB-PN893)
Related Product 4: NF-H S532 phosphorylation antibody (Cat. No.: AB-PN970)
Related Product 5: N-FH S606 phosphorylation antibody (Cat. No.: AB-PN971)
Related Product 6: N-FH S648 phosphorylation antibody (Cat. No.: AB-PN972)
Scientific Background: NF-L (NEFL; neurofilament light chain) is a 61-68 kDa cytoskeletal protein that belongs to the intermediate filament family and a structural protein in axons. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three. It is essential for maintaining axonal caliber, shape, and stability. It likely cooperates with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks, which are essential for axon development, neurotransmission, and radial axon diameter. It features a tripartite structure (a head, a central alpha-helical rod domain, and a tail) that allows it to polymerize and provide structural integrity. It is found in the cytoplasm, axon, and neuron projections. NF-L can serve as a sensitive blood and cerebrospinal fluid (CSF) biomarker for neuroaxonal damage that is significantly elevated in amylotrophic lateral sclerosis (ALS) patients, often preceding symptoms by up to 12 months. It is used to diagnose, predict progression rates, and assess prognosis, with higher levels correlating to faster, more severe disease. Elevated serum/CSF levels of NF-L also indicate neuronal injury in other neurological diseases like Multiple Sclerosis (MS), Alzheimer's, Parkinson’s, and traumatic brain injury (TBI).This description may include information annotated by UniProt and/or Google AI.