Product Name:
GALNT7
Product Number:
ab-nn463-1
Target Full Name: N-acetylgalactosaminyltransferase 7
Target Alias: Polypeptide GalNAc transferase 7
Product Type Specific: GALNT7 pan-specific antibody
Antibody Code: NN463-1
Antibody Target Type: Pan-specific
Protein UniProt: Q86SF2
Protein SigNET: GALNT7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human GALNT7
Antibody Immunogen Sequence: LESIRRINKAKNEQEHHAGC
Antibody Immunogen Description: Corresponds to amino acid residues L82 to G100.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Scientific Background: GALNT7 (N-acetylgalactosaminyltransferase 7) is a lycopeptide transferase involved in O-linked oligosaccharide biosynthesis. This enzyme catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide as outlind in the following reaction: L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H+. Unike many other proteins of this family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Some peptide transferase activity is possible, considering that its appropriate peptide substrate may remain unidentified. It is widely expressed across various human tissues, including the brain, kidney, and digestive tract. It is primarily located in the membrane of the Golgi apparatus, consistent with glycosylation function. It is often targeted by microRNAs (e.g., miR-30c, miR-34a, miR-494) to regulate its levels, which when elevated, promote proliferation and metastasis. Overexpression of GALNT7 is associated with cancer progression and poor prognosis in various malignancies, including prostate cancer, glioma, and colorectal cancer. There is a positive correlation between GALNT7 and immune-related genes in certain cancers, such as Hepatocellular carcinoma (LIHC) and glioma (GBM), but negative correlations in breast cancer (BRCA). This description may include information annotated by UniProt and/or Google AI.