Product Name:
SAA2-3
Product Number:
ab-nn460-3
Target Full Name: Serum amyloid A-2 protein
Product Type Specific: SAA2 pan-specific antibody
Antibody Code: NN460-3
Antibody Target Type: Pan-specific
Protein UniProt: P0DJI9
Protein SigNET: SAA2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human SAA2
Antibody Immunogen Sequence: CGRSGRDPNHFRPAGLPEKY
Antibody Immunogen Description: Corresponds to amino acid residues G104 to Y122.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Scientific Background: SAA2 (Serum Amyloid A2) is a major, highly inducible acute-phase protein primarily synthesized by the liver in response to inflammation and tissue injury. It belongs to the SAA family. As a 12 kDa apolipoprotein, it associates with high-density lipoprotein (HDL) in plasma, it plays a key role in cholesterol metabolism and immune response. SAA2 is a key component of the acute phase response, with expression increasing up to 1000-fold during inflammation, driven by cytokines such as IL-6 and TNF-alpha. It functions in HDL metabolism by displacing apolipoprotein A1, which can affect cholesterol transport and promote inflammation. It is expressed in the liver, and secreted into plasma. It can form amyloid fibrils, particularly under acidic conditions, and has been identified as a biomarker for certain cancers and inflammation. High levels of SAA2 are associated with atherosclerosis, rheumatoid arthritis, Crohn's disease, amyloidosis and Alzheimer's disease. In mice, SAA2 overexpression is linked to amyloidosis and metastasis. This description may include information annotated by UniProt and/or Google AI.