Product Name:
14-3-3-pan-1
Product Number:
ab-nn441-2
Target Full Name: 14-3-3 protein beta/alpha (cross-reacts with other isoforms)
Target Alias: 1433B; 143B; KCIP-1; Protein 1054; Protein kinase C inhibitor protein-1; YWHAB
Product Type Specific: 14-3-3b (YWHAB) pan-specific antibody
Antibody Code: NN441-2
Antibody Target Type: Pan-specific
Protein UniProt: P31946
Protein SigNET: 14-3-3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human 14-3-3-1
Antibody Immunogen Sequence: QKAKLAEQAERYDDMAC
Antibody Immunogen Description: Corresponds to amino acid residues Q8 to A23 of 14-3-3-beta .
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.44 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Nematode worm | Budding yeast.
Related Product 1: 14-3-3 expression antibody (Cat. No.: AB-NN441-3)
Scientific Background: 14-3-3 beta is a protein of the 14-3-3 family of proteins implicated in the regulation of a large spectrum of both general and specialized signalling pathways. It binds to a large number of partners through recognition of a phosphoserine or phosphothreonine motif. Its binding generally results in the modulation of the activity of the binding partner. 14-3-3 beta is a negative regulator of osteogenesis. It blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression, which results in blockage of neuronal apoptosis elicited by SRPK2. It is a negative regulator of signalling cascades that mediate activation of MAP kinases via AKAP13. This description may include information annotated by UniProt and/or Google AI.