Product Name:
CASR
Product Number:
ab-nn382-1
Target Full Name: Extracellular calcium sensing receptor
Target Alias: Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism; HHC; FIH; Parathyroid Cell calcium-sensing receptor; CASR; CASR_HUMAN; Ca sensing receptor; Extracellular calcium-sensing receptor; PCAR1; FHH; Ca2+ sensing receptor; EIG8; Hypocalciuric hypercalcemia 1; PCaR1; GPRC2A; Extracellular calcium sensing receptor [Precursor]; PCAR 1; Parathyroid cell calcium-sensing receptor 1; HHC1; Parathyroid Cell calcium sensing receptor; CAR; MGC138441; NSHPT; CaSR; Ca2+ sensing receptor 1; Parathyroid Ca(2+) sensing receptor 1;
Product Type Specific: G protein-coupled receptor pan-specific antibody
Antibody Code: NN382-1
Antibody Target Type: Pan-specific
Protein UniProt: P41180
Protein SigNET: CASR
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human calcium-sensing receptor (CASR)
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a weak ~135 kDa protein band in rat kidney lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Positive Control: A 1:1000 dilution of SPC-713 was sufficient for detection of calcium-sensing receptor (CASR) in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain and rat kidney cell lysates.
Scientific Background: CASR (Extracellular calcium-sensing receptor) is a 7-transmembrane G protein-coupled receptor (7TM GPCR) belongs to the G protein-coupled receptor 3 family. It senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis. It monitors fluctuations in the circulating calcium concentration and modulates the production of parathyroid hormone (PTH) in parathyroid glands. The activity of this receptor is mediated by a G protein that activates a phosphatidylinositol-calcium second messenger system. CASR is coupled with different G(q)/G11, G(i)/G(o)- or G(s)-classes of G proteins depending on the context. In the parathyroid and kidney, CASR signals through G(q)/G11 and G(i)/G(o) G proteins: G(q)/G11 coupling activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers, while G(i)/G(o) coupling mediates inhibition of adenylate cyclase activity. The G protein-coupled receptor activity is activated by a co-agonist mechanism: aromatic amino acids, such as Trp or Phe, act concertedly with divalent cations, such as calcium or magnesium, to achieve full receptor activation. CASR is found in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum in the brain, and also expressed in kidney, lung, liver, heart, skeletal muscle, and placenta. Mutations in the CASR gene are linked to calcium-related disorders. Inactivating mutations cause Familial Hypocalciuric Hypercalcemia (FHH) or neonatal severe hyperparathyroidism, while activating mutations lead to autosomal dominant hypocalcemia (ADH). This description may include information annotated by UniProt and/or Google AI.