Product Name:
a-Tubulin (TUBA1B)
Product Number:
ab-nn380-1
Target Full Name: Alpha-tubulin ubiquitous
Target Alias: Alpha tubulin ubiquitous; Tubulin alpha 1B chain; Tubulin alpha ubiquitous; Tubulin K-alpha-1; TBA1B_HUMAN; K alpha 1; Tubulin alpha ubiquitous chain; Tubulin alpha 1B; TUBA1B; Tubulin alpha-1B chain; Tubulin alpha-ubiquitous chain; Tubulin K alpha 1
Product Type Specific: Cytoskeletal protein pan-specific antibody
Antibody Code: NN380-1
Antibody Target Type: Pan-specific
Protein UniProt: P68363
Protein SigNET: a-Tubulin (TUBA1B)
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human alpha-Tubulin
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a strong ~54 kDa protein in mouse brain lysates, and a weak ~50 kDa protein band in HeLa and A549 cell lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Positive Control: A 1:1000 dilution of SPC-692 was sufficient for detection of alpha-Tubulin in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain, HeLa and A549 cell lysates.
Scientific Background: Alpha-Tubulin (Tubulin alpha-1B chain; TUBA1B) is a cytoskeletal protein that belongs to the tubulin family. Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. It facilitates critical processes including cell shape maintenance, intracellular transport, and mitosis. TUBA1B is localized within the microtubule and cilia. As a key component of the cytoskeleton, it is frequently used as a, often, stable "housekeeping" control. However, it is highly expressed in rapidly dividing cells, such as those found in cancerous tumours, including hepatocellular carcinoma (HCC), lung adenocarcinoma, and Wilms' tumour. This description may include information annotated by UniProt and/or Google AI.