Product Name:
Rab1B
Product Number:
ab-nn378-1
Target Full Name: Ras-related protein Rab-1B
Target Alias: RAB1B, member RAS oncogene family
Product Type Specific: G (Rab) protein pan-specific antibody
Antibody Code: NN378-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9H0U4
Protein SigNET: Rab1B
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after the C-terminal of human RAB1B
Antibody Immunogen Sequence: SGGERPNLKID
Antibody Immunogen Description: Corresponds to amino acid residues S179-D189.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium-strong ~48 kDa protein band in HeLa cells by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Platypus | Zebra fish.
Antibody Positive Control: A 1:1000 dilution of SPC-684 was sufficient for detection of RAB1B in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain and HeLa cell lysates.
Related Product 1: Rab5 pan-specific antibody (Cat. No.: AB-NN150)
Scientific Background: Rab1B (Ras-related protein Rab-1B) is a small GTPase that belongs to the small GTPase superfamily, Rab family. Rab proteins are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rab proteins cycle between inactive GDP-bound forms and an active GTP-bound forms that are able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Rab1 functions in the initial events of the autophagic vacuole development that take place at specialized regions of the endoplasmic reticulum and proceeds through successive Golgi compartments. It is required to modulate the compacted morphology of the Golgi. The GTP bound to Rab proteins undergoes degradation through the intrinsic GTPase activity of Rab proteins. This description may include information annotated by UniProt and/or Google AI.