Product Name:
IFT88 (TTC10)
Product Number:
ab-nn377-1
Target Full Name: Tetratricopeptide repeat domain 10
Target Alias: D13S1056E; TPR repeat protein 10; Ift88; Recessive polycystic kidney disease protein Tg737 homologue; IFT88_HUMAN; Polaris homologue; Probe hTg737 (polycystic kidney disease; autosomal recessive); TG737; TTC10; DAF19; IFT88; hTg737; Intraflagellar transport 88 homologue; MGC26259; Tetratricopeptide repeat protein 10; Intraflagellar transport protein 88 homologue; RP11-172H24.2
Product Type Specific: IFT88 pan-specific antibody
Antibody Code: NN377-1
Antibody Target Type: Pan-specific
Protein UniProt: Q13099
Protein SigNET: IFT88 (TTC10)
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after the mid to C-terminal of human IFT88
Antibody Immunogen Sequence: LDTYKDTHRKFPE
Antibody Immunogen Description: Corresponds to amino acid residues L674-E686.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium ~94 kDa protein band in A549 cells by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Zebra fish.
Antibody Positive Control: A 1:1000 dilution of SPC-679 was sufficient for detection of IFT88 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain and A549 cell lysates.
Scientific Background: IFT88 (Intraflagellar transport protein 88 homolog; polaris) is a 93 kDa protein in the IFT Complex B essential for cilia assembly, maintenance, and signalling (e.g., in the Hedgehog signaling pathway). It appear to be involved in primary cilium biogenesis, and is also implicated in autophagy, since it is required for trafficking of ATG16L and the expansion of the autophagic compartment. It is a tetratricopeptide (typically 12) repeat (TPR) protein, through which it is likely involved in multiple protein-protein interactions. It interacts with other IFT complex B proteins, such as IFT20, IFT74, and IFT81. Beyond cilia, IFT88 has roles in cell-cycle regulation (G1-S transition), mitosis, and mediating cell polarization during migration. It is expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas. It is located in the cytoplasm, centrosomes, cilia basal bodies, and along the cilia body. Mutations in this region are linked with defective cilia, ciliopathies, polycystic kidney disease (PKD), retinal degeneration, and left-right asymmetry. This description may include information annotated by UniProt and/or Google AI.