Product Name:
FIP200 (RB1CC1)
Product Number:
ab-nn376-1
Target Full Name: RB1-inducible coiled-coil protein 1
Target Alias: RB1CC1; FAK family kinase-interacting protein of 200 kDa; FIP200; KIAA0203; RBICC
Product Type Specific: FIP200 pan-specific antibody
Antibody Code: NN376-1
Antibody Target Type: Pan-specific
Protein UniProt: Q8TDY2
Protein SigNET: FIP200 (RB1CC1)
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after the C-terminal of human FIP200
Antibody Immunogen Sequence: KSTMQQQERDKD
Antibody Immunogen Description: Corresponds to amino acid residues K1355-D1366.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium ~150 kDa protein band in rat brain lysates and ~190 kDa protein in mouse brain lysates.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus.
Antibody Positive Control: A 1:1000 dilution of SPC-658 was sufficient for detection of FIP200 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: No other prominent cross-reactive proteins in rat and mouse brain lysates.
Scientific Background: FIP200 (RB1CC1) is a transcription factor that belongs to the ATG17 family. FIP200 is involved in autophagy, and regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1. It is required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S. typhimurium infection and subsequent xenophagy. It is involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis. IFIP200 inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways. It plays roles as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111, in muscular differentiation, and in fetal hematopoiesis and in the regulation of neuronal homeostasis. It is also a potent regulator of the RB1 pathway through induction of RB1 expression. Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. It is abundantly expressed in human musculoskeletal and cultured osteosarcoma cells. This description may include information annotated by UniProt and/or Google AI.