Product Name:
SQSTM1
Product Number:
ab-nn375-1
Target Full Name: Sequestosome-1
Target Alias: EBI3-associated protein of 60 kDa; ORCA; Osi; SQSTM 1; ZIP 3; A170; SQSTM1; Ubiquitin binding protein p62; Protein kinase C-zeta-interacting protein; FTDALS3; Ubiquitin-binding protein p62; PKC-zeta-interacting protein; EBIAP; Sqstm1; Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa; EBI3 associated protein p60; EBI3 associated protein of 60 kDa; SQSTM_HUMAN; OSF-6; Paget disease of bone 3; p62B; p60; PDB 3; EBI 3 associated protein p60; MGC127197; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; EBI 3 associated protein of 60 kDa; ZIP3; STAP; p62; Sequestosome 1; OSIL; STONE14; Phosphotyrosine independent ligand for the Lck SH2 domain p62; PDB3; Oxidative stress induced like; ZIP
Product Type Specific: SQSTM1 adapter/scaffold protein pan-specific antibody
Antibody Code: NN375-1
Antibody Target Type: Pan-specific
Protein UniProt: Q13501
Protein SigNET: SQSTM1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after the C-terminal of human SQSTM1
Antibody Immunogen Sequence: DDDWTHLSSKE
Antibody Immunogen Description: Corresponds to amino acid residues D335-E345.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects weak-medium ~62 and 40 kDa isoform bands in mouse brain lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus.
Antibody Positive Control: A 1:1000 dilution of SPC-637 was sufficient for detection of SQSTM1 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain and Jurkat cell lysates.
Related Product 1: SQSTM1 S403 phosphorylation antibody (Cat. No.: AB-PN908)
Related Product 2: SQSTM1 T269+S272 phosphorylation antibody (Cat. No.: AB-PN909)
Related Product 3: SQSTM1 T339+S342 phosphorylation antibody (Cat. No.: AB-PN910)
Scientific Background: SQSTM1 (Sequestome 1 (p62)) is a ubiquitin binding protein that interacts with ubiqutin, providing a scaffold for several signalling proteins and triggering degradation of proteins throught the proteasome or lysosome. It functions as a bridge between polyubiquitinated cargo and autophagosomes. It regulates activation of the nuclear factor kappa-B (NF-kB) signalling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. SQSTM1 also binds autophagosomal membrane protein LC3/ATG8. Mutations in the SQSTM1 gene result in sporadic and familial Paget disease of bone, and have been linked with amyotrophic lateral sclerosis (ALS). This description may include information annotated by UniProt and/or Google AI.