Product Name:
ATG4B
Product Number:
ab-nn374-1
Target Full Name: Autophagy-related cysteine endopeptidase 1
Target Alias: MGC1353; ATG4B; hAPG4B; AUT-like 1 cysteine endopeptidase; Cysteine protease ATG4B; Autophagy-related protein 4 homologue B; Autophagin-1; EC:3.4.22.-; ATG4B_HUMAN; APG4B; APG4 autophagy 4 homologue B; AUTL1; KIAA0943; Autophagy related cysteine endopeptidase 1; Autophagin 1; ATG4 autophagy related 4 homologue B (S. cerevisiae); AUT like 1 cysteine endopeptidase; Autophagy related protein 4 homologue B;
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN374-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y4P1
Protein SigNET: ATG4B
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after the N-terminal to mid of human ATG4B
Antibody Immunogen Sequence: RDWRWTQRKRQPD
Antibody Immunogen Description: Corresponds to amino acid residues R94-D106.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium ~44 kDa protein band in mouse brain lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse.
Antibody Positive Control: A 1:1000 dilution of SPC-630 was sufficient for detection of ATG4B in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain lysates.
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Scientific Background: Autophagy related 4B, cysteine peptidase (ATG4B or APG4B) is a C-54 cysteine protease that also belongs to the family of autophagin poteins. Specifically it cleaves LC3, and mutations can be associated with strong inhibition of autophagosome formation. It is responsible for processing and recycling ATG8-family proteins (like LC3 and GABARAP). It also acts as a delipidating enzyme to recycle these proteins. It activates LC3 and GABARAP by exposing a C-terminal glycine required for conjugation to phosphatidylethanolamine (PE). Its activity is regulated by phosphorylation, specifically at residues Ser-34, Ser-383, and Ser-392, which enhances its binding to LC3. ATG4B is a potential target for anti-cancer treatments, as it is involved in cancer progression and regulation of cell homeostasis. This description may include information annotated by UniProt and/or Google AI.