Product Name:
ATG4A
Product Number:
ab-nn373-1
Target Full Name: ATG4 autophagy related 4 homologue A (S. cerevisiae)
Target Alias: MGC107179; AV169859; Autophagy-related cysteine endopeptidase 2; hAPG4A; EC:3.4.22.-; ATG4A; Autophagin 2; Autophagy related protein 4 homologue A; Cysteine protease ATG4A; AUTL2; Autl2; Autophagy-related protein 4 homologue A; Atg4al; Autophagin-2; Autophagy related 4A cysteine peptidase; AUT like 2 cysteine endopeptidase; Autophagy related cysteine endopeptidase 2; Apg4a; AUT-like 2 cysteine endopeptidase; APG4A; AI627006; ATG4A_HUMAN;
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN373-1
Antibody Target Type: Pan-specific
Protein UniProt: Q8WYN0
Protein SigNET: ATG4A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after the mid-protein of human ATG4A
Antibody Immunogen Sequence: EKQKEQPKEYQR
Antibody Immunogen Description: Corresponds to amino acid residues E102-R113.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium ~45 kDa protein band in mouse brain lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Antibody Positive Control: A 1:1000 dilution of SPC-628 was sufficient for detection of ATG4A in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain lysates.
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Scientific Background: Autophagy related 4A, cysteine peptidase (ATG4A or APG4A) is a C-54 cysteine protease that also belongs to the family of autophagin proteins, and is primarily found in skeletal muscle and brain. ATG4A mediates cell remodeling and homeostasis, and is often involved in cancer development and cellular stress responses. It has a high preference for GABARAPL2, followed by MAP1LC3A and GABARAP. ATG4A primes pro-LC3 and GABARAP proteins by cleaving their C-termini to expose a glycine residue necessary for their membrane insertion It cleaves ATG8 family proteins to enable their conjugation to phosphatidylethanolamine, a crucial step for autophagosome formation. It cleaves GATE-16. Mutations in the ATG4a gene is critical for redox regulation and inhibits autophagosome formation. ATG4A is important for maintaining a stem-like phenotype in breast cancer cells. It has also been linked to promoting tumour metastasis in other contexts. TG4A may be a potential target in cataract therapies in view of its role in lens degradation. This description may include information annotated by UniProt and/or Google AI.