Product Name:
Beclin 2 (BECN1L1)
Product Number:
ab-nn372-2
Target Full Name: Beclin-1-like protein 1
Target Alias: BECN1P1; Beclin-1 autophagy-related pseudogene 1; BECN2; Beclin-2; BECN1L1; Becn2; Beclin-2; PFM 2; PR domain zinc finger protein 5; PRDM 5; PR domain-containing protein 5; PRDM5; PRDM5_HUMAN; PR domain containing protein 5; BCS2
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN372-2
Antibody Target Type: Pan-specific
Protein UniProt: A8MW95
Protein SigNET: Beclin 2 (BECN1L1)
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after the C-terminal of mouse Beclin 2 (Uniprot ID O88597)
Antibody Immunogen Sequence: SYSIKTQFNSEE
Antibody Immunogen Description: Corresponds to amino acid residues S410-E421.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a weak ~48 kDa protein band in mouse brain lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Platypus | Chicken | Frog | Tiger blowfish | Fruit fly | Honey bee | Sea urchin.
Antibody Positive Control: A 1:1000 dilution of SPC-604 was sufficient for detection of Beclin 2 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain lysates.
Related Product 1: Beclin 2 (BECN1L1) expression antibody (Cat. No.: AB-NN372-1)
Related Product 2: Beclin 1 expression antibody (Cat. No.: AB-NN217-1)
Related Product 3: Beclin 1 expression antibody (Cat. No.: AB-NN217-2)
Scientific Background: Beclin 2 is a 49 kDa novel coiled-coil protein related to the autophagic Beclin 1 protein. It features a BH3 domain, a coiled-coil domain, and a C-terminal domain. It functions in two distinct lysosomal degradation pathways: autophagy and the degradation of G protein-coupled receptors (GPCRs) via interaction with GASP1. It is thought to interact with Bcl-2, an anti-apoptotic protein. It has also been found to mediate G protein-coupled receptor (GPCR) degradation (1). While both Beclin 1 and Beclin 2 regulate autophagy, Beclin 2 uniquely facilitates the agonist-induced lysosomal degradation of specific GPCRs (such as CB1R and DOR). Becilin 2 acts as a negative regulator of innate immune signalling, and mediates the degradation of inflammasome sensors such as AIM2 and NLRP3. It is predominantly expressed in the brain, heart, skeletal muscle, placenta, and uterus, with distinct expression patterns from Beclin 1. Beclin 2 is involved in metabolism, as haploinsufficiency (loss of one allele) in mice results in increased food intake, obesity, and insulin resistance, partly due to reduced degradation of the cannabinoid receptor 1 (CB1R). This description may include information annotated by UniProt and/or Google AI.