Product Name:
Alpha-2C Adrenergic Receptor
Product Number:
ab-nn371-2
Target Full Name: Alpha-2C adrenergic receptor
Target Alias: Adra 2c; Alpha-2CAR; ADRA2C; ADRA2L2; ADRARL2; Alpha 2CAR; alpha2 AR C4; alpha2 C4; alpha2C; ALPHA2CAR; Alpha-2 adrenergic receptor subtype C4; Alpha-2C adrenoreceptor; Adrenergic alpha 2C receptor; Alpha 2C adrenergic receptor subtype C4; Alpha 2C adrenoceptor; Alpha 2C adrenoreceptor; Alpha-2-adrenergic receptor; renal type; alpha2cii-adrenergic receptor; Subtype alpha2 C4
Product Type Specific: G protein-coupled receptor pan-specific antibody
Antibody Code: NN371-2
Antibody Target Type: Pan-specific
Protein UniProt: P18825
Protein SigNET: Alpha2C Adrenergic Receptor
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide patterned after cytoplasmic C-terminus of human Alpha-2C adrenergic receptor
Antibody Immunogen Sequence: QDFRRSFKHILFRRRRRGFRQ
Antibody Immunogen Description: Corresponds to amino acid residues Q442- Q462.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a 50 kDa protein or larger (possibly due to dimerization).
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-435 was sufficient for detection of Alpha2C Adrenergic Receptor in 20µg of COS cells transiently transfected with HA-tagged Alpha- lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with other adrenergic receptors.
Related Product 1: Alpha-2C Adrenergic Receptor pan-specific antibody (Cat. No.: AB-NN371-1)
Scientific Background: The alpha 2C Adrenergic Receptor (a2C-AR) is a G protein-coupled receptor (GPCR) that mediates sympathetic nervous system activity. It functions primarily as a presynaptic autoreceptor, regulating neurotransmitter release at low nerve activity levels. It is predominantly found in the central nervous system, particularly the basal ganglia, and plays a role in cold-induced vasoconstriction. a2C-AR controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. It inhibits norepinephrine release in the central nervous system and sympathetic nerves. This receptor also plays a role in cognitive and behavioral function. It acts as an inhibitory receptor, coupled with Gi/o proteins to inhibit adenylyl cyclase, which suppresses cAMP production. It promotes ERK1/2 stimulation and suppresses voltage-gated calcium channels. It features 7-transmembrane helical bundle (TM I–VII), three extracellular loops (ECL1–3), three intracellular loops (ICL1–3), and a C-terminal helix VIII. Two variants are produced by alternative splicing. a2C-AR has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein. It affects the regulation of sympathetic adrenergic stimulation and plays a role in the pathogenesis of conditions like Raynaud's phenomenon. It is implicated in psychiatric conditions, such as schizophrenia or bipolar disorder. This description may include information annotated by UniProt and/or Google AI.