Product Name:
TLR4
Product Number:
ab-nn368-1
Target Full Name: Toll-like receptor 4
Target Alias: ARMD10; CD284; TLR 4; TOLL
Product Type Specific: Toll like receptor pan-specific antibody
Antibody Code: NN368-1
Antibody Target Type: Pan-specific
Protein UniProt: O00206
Protein SigNET: TLR4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after human TLR4
Antibody Immunogen Sequence: GLEQLEHLDFQHSNLK
Antibody Immunogen Description: Corresponds to amino acid residues G420-K435.
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 0.05% BSA, 0.05% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | FACS
Antibody Dilution Recommended: WB (1:500), IHC (1:50); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium-high potency. Detects a ~75-80 kDa protein when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-high
Scientific Background: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signalling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6). This description may include information annotated by UniProt and/or Google AI.