Product Name:
VGLUT1
Product Number:
ab-nn361-1
Target Full Name: Vesicular glutamate transporter 1
Target Alias: BNPI; SLC17A1; SLC17A1/VGLUT1; Solute Carrier family 17 member 7; VGLUT 1; Brain-specific Na(+)-dependent inorganic phosphate cotransporter
Product Type Specific: Solute carrier protein pan-specific antibody
Antibody Code: NN361-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9P2U7
Protein SigNET: Q9P2U7
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
Antibody Modification: Solute carrier protein pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~52 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-394 was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: No cross-reactivity against VGlut2.
Related Product 1: VGLUT2 pan-specific antibody (Cat. No.: AB-NN323-1)
Related Product 2: VGLUT3 pan-specific antibody (Cat. No.: AB-NN362-1)
Scientific Background: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).