Product Name:
Versican
Product Number:
ab-nn360-1
Target Full Name: Versican core protein
Target Alias: Chondroitin sulfate proteoglycan 2; CSPG2; ERVR; GHAP; PG-M; VCAN; Chondroitin sulfate proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; Glial hyaluronate binding protein; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; Large fibroblast proteoglycan; PGM; V1 Neo; Versican proteoglycan; Versican V0; WGN 1; WGN; WGN1
Product Type Specific: Versican pan-specific antibody
Antibody Code: NN360-1
Antibody Target Type: Pan-specific
Protein UniProt: P13611
Protein SigNET: Versican
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with glycosaminoglycan alpha domain of mouse Versican core protein (Uniprot ID Q62059)
Antibody Immunogen Description: Corresponds to amino acid residues I362-I585.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a >350 kDa protein by Western blotting.
Antibody Species Reactivity: Rat | Mouse
Antibody Positive Control: 1 µg/ml of SMC-439 was sufficient for detection of Versican in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a Lectinlike sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length anddegree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in lesions of athero-sclerosis suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumour development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may faciliatate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels. This description may include information annotated by UniProt and/or Google AI.