Product Name:
VAMP1/2
Product Number:
ab-nn358-1
Target Full Name: Vesicle-associated membrane protein 1
Target Alias: SYB1; Synaptobrevin; Synaptobrevin-1; vesicle associated membrane protein
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN358-1
Antibody Target Type: Pan-specific
Protein UniProt: P23763
Protein SigNET: VAMP1/2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Crude human synaptic immunoprecipitate
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ELISA
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~20-25 kDa proteins. Recognizes VAMP 1 and 2.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat.
Antibody Positive Control: 1 µg/ml of SMC-179 was sufficient for detection of VAMP in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: VAMP1 S63 phosphorylation antibody (Cat. No.: AB-PN1001)
Related Product 2: VAMP4 S30 phosphorylation antibody (Cat. No.: AB-PN1002)
Scientific Background: VAMP1 (vesicle-associated membrane protein) is an 18 kDa member of the synaptobrevin family of proteins. It is an integral membrane protein crucial for neurotransmitter release and vesicle fusion at the presynaptic nerve terminal. It acts as a key component of the SNARE complex, mediating docking and exocytosis of synaptic vesicles. It participates in vesicle fusion with the plasma membrane (1). Synaptobrevins, Syntakins and the 25 kDa synaptosomal associated SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane (2). VAMP-1 is also one of the key proteins in the SNARE complex which is involved in regulated exocytosis (3). Primarily found in the nervous system (specifically brain and spinal cord) and neuromuscular junctions, with some isoforms expressed in other tissues (e.g., kidney, exocrine pancreas). Multiple splice variants exist, with VAMP1A (primarily neural) and VAMP1B (broadly expressed) being common. Mutations in VAMP1 are linked to serious disorders such as congenital myasthenic syndromes and hereditary spastic ataxia, resulting from weakened neurotransmitter release. VAMP1 is a target for botulinum neurotoxin serotype D, which cleaves the protein, causing neuromuscular paralysis. This description may include information annotated by UniProt and/or Google AI.