Product Name:
VAChT
Product Number:
ab-nn357-1
Target Full Name: Vesicular acetylcholine transporter
Target Alias: Vesicular Acetylcholine Transporter; MGC12716; rVAT; Slc18a3; Solute carrier family 18 (vesicular acetylcholine) member 3; Solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3
Product Type Specific: Solute carrier protein pan-specific antibody
Antibody Code: NN357-1
Antibody Target Type: Pan-specific
Protein UniProt: Q16572
Protein SigNET: VAChT
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide patterned after human VAChT
Antibody Immunogen Sequence: CEDDYNYYYTRS
Antibody Immunogen Description: Corresponds to amino acid residues C521-S532.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~56 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: A dilution of 1:50-1:200 of SMC-341 was sufficient for detection of VAChT Transporter in rat brain using immunohistochemistry analysis and goat anti-mouse IgG:HRP as the secondary antibody.
Scientific Background: VAChT is a 75 kDa member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicle for release into the extracellular space. Acetylcholine (Ach) transport utilizes a proton gradient established by a vacuolar ATPase. It acts as an antiporter, swapping vesicle protons (H+) for acetylcholine, which is crucial for cholinergic transmission and neuroregulation. It is expressed in cholinergic neurons and specialized non-neuronal cells (e.g., airway epithelia). The VAChT gene is uniquely "nested" within the first intron of the choline acetyltransferase (ChAT) gene. This description may include information annotated by UniProt and/or Google AI.