Product Name:
UVRAG
Product Number:
ab-nn356-1
Target Full Name: UV radiation resistance-associated gene protein
Target Alias: DHTX; p63; UV radiation resistance associated; UVRAG_HUMAN
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN356-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9P2Y5
Protein SigNET: UVRAG
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after the C-terminal of human UVRAG
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Predicted molecular mass at ~78.1 kDa protein in cell and tissue lysates by Western blotting. Observed molecular masses in the 75-90 kDa protein range.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Specificity: High
Antibody Cross Reactivity: Two other cross-reactive proteins of ~50 and ~25 kDa in mouse brain lysates.
Related Product 1: UVRAG pan-specific antibody (Cat. No.: AB-NN356-2)
Scientific Background: UVRAG (UV radiation resistance-associated gene protein) is a versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. It regulates COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex, in a manner dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P). During autophagy, it acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is thought to be involved in maturation of autophagosomes and endocytosis. It stimulates the lipid kinase activity of PIK3C3. It is involved in the regulation of degradative endocytic trafficking and cytokinesis, and in the regulation of ATG9A transport from the Golgi to the autophagosome. it is involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depends on its association with a class C Vps complex (possibly the HOPS complex). The association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events. Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion. In case of negative-strand RNA virus infection, it is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly. It is also involved in maintaining chromosomal stability. It promotes DNA double-strand break (DSB) repair by association with DNA-dependent protein kinase complex DNA-PK and activating it in non-homologous end joining (NHEJ). It is required for centrosome stability and proper chromosome segregation. UVRAG is highly expressed in brain, lung, kidney and liver. This description may include information annotated by UniProt and/or Google AI.