Product Name:
Trak2
Product Number:
ab-nn348-1
Target Full Name: Trafficking kinesin-binding protein 2
Target Alias: Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein; GABA-A receptor-interacting factor 1; O-GlcNAc transferase-interacting protein of 98 kDa; EMBL AAH60681.1; Ensembl ENSMUSP00000027186 MGI 1918077; Als2cr3; Grif1; Oip98; KIAA0549; TRAK2_Human
Product Type Specific: Trak2 pan-specific antibody
Antibody Code: NN348-1
Antibody Target Type: Pan-specific
Protein UniProt: O60296
Protein SigNET: Trak2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2A
Antibody Immunogen Source: Fusion protein with peptide from C-terminus) of human TRAK2
Antibody Immunogen Description: Corresponds to amino acid residues S757-D914.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~100 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat.
Antibody Positive Control: A 1:100 dilution of SMC-483 was sufficient for detection of Trak2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with TRAK1.
Scientific Background: TRAK2 (Trafficking kinesin-binding protein 2), a member of the TRAK family of adaptor proteins.. It is a mitochondrial adaptor protein that regulates intracellular transport by linking mitochondria to microtubule motors (kinesin and dynein). It is localized in dendrites, it is specifically required for dendritic development as it reacts with dynein and dynactin. It serves as a molecular adaptor, acting as a bridge between mitochondrial membrane receptors (like Miro) and microtubule motors.TRAK2 preferentially interacts with the dynein/dynactin complex to drive transport toward the microtubule minus-end (dendritic transport). However, it can activate both motors. It is primarily localized in dendrites of hippocampal neurons, though it is found in both axons and dendrites in cortical neurons. TRAK2 interferes with kinesin-1 binding and axonal transport (1). It has been shown to interact with Kir2.1 and GABA-R Beta2 (2, 3). Beyond mitochondrial transport, it has been implicated in GABA receptor regulation, intracellular vesicle transport, and regulation of cholesterol efflux. TRAK2 is involved in neuronal homeostasis, and its dysfunction can affect mitochondrial distribution. It has been associated with neurological conditions, including studies identifying it in the amyotrophic lateral sclerosis 2 (juvenile) chromosome region. This description may include information annotated by UniProt and/or Google AI.