Product Name:
TNF-R1
Product Number:
ab-nn347-1
Target Full Name: Tumour necrosis factor receptor superfamily member 1A
Target Alias: Tumour necrosis factor receptor 1; TNFR-1; TNFRSF1A; TNFAR; TNFR1
Product Type Specific: TNFR pan-specific antibody
Antibody Code: NN347-1
Antibody Target Type: Pan-specific
Protein UniProt: P19438
Protein SigNET: TNFR1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide from near the N-terminus of mouse TNF-R1 (Uniprot ID P25118)
Antibody Immunogen Sequence: MGIHPSGVTGLVPSLGDREKRDSL
Antibody Immunogen Description: Corresponds to amino acid residues M20-L43. Identical to rat and human over those residues.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.09% azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~55 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Monkey | Cow | Dog | Rabbit | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SPC-170 was sufficient for detection of TNFR1 in 20 µg of Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP asthe secondary antibody.
Antibody Specificity: Medium
Antibody Cross Reactivity: Antibody seems to have a generally high background. in mouse brain lysates.
Scientific Background: The tumour necrosis factor receptor (TNFR) also known as Cluster of differentiation (CD120) is a protein that belongs to the (TNF)/ (TNFR) superfamily. TNF interacts with two distinct receptors TNFR1 and TNFR2. These receptors share no homology on their cytoplasmic sequences(1,3).TNFR1 also known as p55/p60 is a high affinity receptor for TNF-α. The TNFR1 has an extracellular domain with variable numbers of cysteine-rich repeats. The functional properties of TNFR1 are targets in new therapies for osteoporosis, chronic inflammatory and autoimmune diseases (1, 2). The TNF-α/TNFR1 receptor complex is responsible for the recruitment and the subsequent activation of the caspase (aspartate-specific cysteine proteases) that regulate apoptosis. This description may include information annotated by UniProt and/or Google AI.