Product Name:
beta 4 Spectrin
Product Number:
ab-nn329-2
Target Full Name: Spectrin beta chain, non-erythrocytic 4
Target Alias: BetaIV-spectrin sigma1; Non-erythrocytic spectrin beta chain 4; Non-erythroidspectrin beta chain 3; SPTBN4; SPTBN3; Spnb4; KIAA1642; EMBL BAB83243.1; Ensembl ENSRNOP00000028393
Product Type Specific: Cytoskeletal protein pan-specific antibody
Antibody Code: NN329-2
Antibody Target Type: Pan-specific
Protein UniProt: Q9H254
Protein SigNET: beta 4 Spectrin
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with C-terminal repeats (14 to 15) of human Beta4-spectrin
Antibody Immunogen Description: Corresponds to amino acid residues F1621-A1832.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a >200 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: A 1:100 dilution of SMC-484 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: Does not cross-react with other Beta-spectrins One very strong ~75 kDa immunoreactive protein.
Scientific Background: Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart (1). Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier (2). This description may include information annotated by UniProt and/or Google AI.