Product Name:
SNAP-25
Product Number:
ab-nn327-1
Target Full Name: Synaptosomal-associated protein 25
Target Alias: Bdr; RIC4; SNAP25; SNAP-25B; SNP25; SUP; super protein; synaptosomal associated 25kDa protein
Product Type Specific: SNAP25 synaptic protein pan-specific antibody
Antibody Code: NN327-1
Antibody Target Type: Pan-specific
Protein UniProt: P60880
Protein SigNET: SNAP
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Crude synaptic preparation from the post mortem human brain
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | ELISA
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:1000) ELISA (1:5000); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~29 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Pig | Cat | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-180 was sufficient for detection of SNAP-25 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Related Product 1: SNAP25 S28+T29 phosphorylation antibody (Cat. No.: AB-PN1003)
Related Product 2: SNAP25 S187 phosphorylation antibody (Cat. No.: AB-PN990)
Related Product 3: SNAP25 T138 phosphorylation antibody (Cat. No.: AB-PN991)
Scientific Background: SNAP25 (Synaptosomal-associated protein 25) is a 25 kDa membrane bound protein anchored to the cytosolic face of membrane via palmitoyl side chains in the middle of the molecule (1). It functions in the regulation of neurotransmitter release, acting as a key component of the SNARE complex to facilitate synaptic vesicle fusion at the presynaptic plasma membrane. It is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together (2). It forms a core four alpha-helix bundle with syntaxin-1 and synaptobrevin (VAMP) to drive the docking and fusion of vesicles with the membrane. SNAP25 exists as two alternatively spliced isoforms, SMAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and they differ by 9 amino acids in the central portion of the protein (3). SNAP25 is anchored to the cytoplasmic face of the plasma membrane via palmitoylated cysteine residues, rather than a transmembrane domain. Mutations or imbalances in SNAP25 are associated with epilepsy, intellectual disability (SNAP25-DEE), and neuropsychiatric disorders like ADHD. Botulinum Toxin Type A (BoNT-A) specifically cleaves SNAP-25, blocking acetylcholine release and inhibiting muscle contraction. This description may include information annotated by UniProt and/or Google AI.