Product Name:
NHE3
Product Number:
ab-nn326-1
Target Full Name: Sodium/hydrogen exchanger 3
Target Alias: SLC9A3; Na+/H+ exchanger 3; isoform 3; Solute carrier family 9 member 3; NHE3MGC126720; MGC126718; solute carrier family 9 (sodium/hydrogen exchanger) member 3; NHE-3; Na(+)/H(+) exchanger 3
Product Type Specific: SLC9A3 solute carrier protein pan-specific antibody
Antibody Code: NN326-1
Antibody Target Type: Pan-specific
Protein UniProt: P48764
Protein SigNET: NHE3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after rat SLC9A3 (Uniprot ID Q9JM15)
Antibody Immunogen Sequence: SFLQADGPEEQLQPASPESTHM
Antibody Immunogen Description: Corresponds to amino acid residues S809 to M831.
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~84 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SPC-400 was sufficient for detection of HNE3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Scientific Background: NHE3 (Sodium-hydrogen exchanger 3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is found in the apical brush border membrane of the proximal tubule in the kidney and the small intestine, and on the basolateral side of pancreatic/duodenal cells. (1). It features an N-terminal membrane domain for transport and a C-terminal regulatory domain. It is highly regulated by the C-terminal cytoplasmic domain, which binds to regulatory proteins (e.g., NHERF1/2) and links to the cytoskeleton. NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2A. It is inhibited by phosphorylation via PKA (activated by PTH/dopamine). It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2). Defects are associated with congenital secretory sodium diarrhea. Reduced function can lead to lower glomerular filtration rates, while its regulation is involved in hypertension and heart failure. This description may include information annotated by UniProt and/or Google AI.