Product Name:
NCC
Product Number:
ab-nn3211
Target Full Name: Solute carrier family 12 member 3
Target Alias: SLC12A3; SCYL1;CKb10; MCP-4; MGC17134; NCC-1; NCC1; SCYA13; CK-beta-10; monocyte chemoattractant protein 4; monocyte chemotactic protein 4; new CC chemokine 1; small inducible cytokine A13; small inducible cytokine subfamily A (Cys-Cys) member 13; chemokine (C-C)
Product Type Specific: Chemokine pan-specific antibody
Antibody Code: NN3211
Antibody Target Type: Pan-specific
Protein UniProt: P55017
Protein SigNET: NCC
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after N-terminus of rat NCC (Uniprot ID P55018)
Antibody Immunogen Sequence: PGEPRKVRPTLADLHSFLKQEG
Antibody Immunogen Description: Corresponds to amino acid residues P74-G95.
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | IEM
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~160 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SPC-402 was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Scientific Background: NCC (SLC12A3), a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It plays a crucial role in blood pressure regulation and ion homeostasis by mediating the reabsorption of sodium (Na+) and chloride (Cl-) from the urine. It is responsible for the reabsorption of roughly 5–10% of the total filtered sodium in the kidney. NCC features 12 membrane-spanning regions with intracellular N- and C-termini. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Phosphorylation is primarily mediated through the WNK-SPAK/OSR1 kinase pathway, which increases its activity. Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1 (1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Gain-of-function mutations lead to Familial Hyperkalemic Hypertension (or Pseudohypoaldosteronism type II). Loss-of-function mutations lead to Gitelman syndrome, characterized by low blood pressure, low potassium, and low magnesium. NCC is the target of thiazide diuretics used in the treatment of hypertension (1). NCC has been detected in the lens cortex, core and fiber cells of a rat (3). This description may include information annotated by UniProt and/or Google AI.