Product Name:
ENaC Beta
Product Number:
ab-nn317-1
Target Full Name: Amiloride-sensitive sodium channel subunit beta
Target Alias: SCNN1B; Amiloride-sensitive sodium channel beta-subunit; Beta ENaC; Beta NaCH; ENaC beta; ENaCB; Epithelial Na(+) channel subunit beta; Epithelial Na+ channel beta subunit; Epithelial Na+ channel subunit beta; Epithelial sodium channel beta 2 subunit; Epithelial sodium channel beta 3 subunit; Nonvoltage gated sodium channel 1 beta subunit; Nonvoltage gated sodium channel 1 subunit beta; Nonvoltage-gated sodium channel 1 beta subunit; SCNEB; SCNN 1B; Sodium channel nonvoltage gated 1 beta (Liddle syndrome); Sodium channel nonvoltage gated 1 beta
Product Type Specific: ENaC Beta pan-specific antibody
Antibody Code: NN317-1
Antibody Target Type: Pan-specific
Protein UniProt: P51168
Protein SigNET: ENaC Beta
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide based on C-terminal tail of rat ENaC beta (Uniprot ID P37090) (antibody designation 3755-2)
Antibody Immunogen Sequence: NYDSLRLQPLDTMESDSEVEAI
Antibody Immunogen Description: Corresponds to amino acid residues N617-I638.
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~87 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog |Rat | Mouse | Platypus.
Antibody Positive Control: 1 µg/ml of SPC-404 was sufficient for detection of beta-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: ENaC Alpha pan-specific antibody (Cat. No.: AB-NN316-1)
Related Product 2: ENaC Gamma pan-specific antibody (Cat. No.: AB-NN369-1)
Scientific Background: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6). This description may include information annotated by UniProt and/or Google AI.