Product Name:
Nav beta 3
Product Number:
ab-nn315-1
Target Full Name: Sodium channel subunit beta-3
Target Alias: Scn3b; KIAA1158
Product Type Specific: Sodium channel pan-specific antibody
Antibody Code: NN315-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9NY72
Protein SigNET: Nav beta 3
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2B
Antibody Immunogen Source: Fusion protein with full-length) of rat NavBeta3 (Uniprot ID Q9JK00)
Antibody Immunogen Description: Corresponds to amino acid residues M1-E215.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~40 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Rat | Mouse.
Antibody Positive Control: A 1:100 dilution of SMC-490 was sufficient for detection of NavBeta3 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-reat with NavBeta1, Navbeta2, or Navbeta4.
Related Product 1: Nav beta 2 pan-specific antibody (Cat. No.: AB-NN314-1)
Related Product 2: Nav1.2 S568 phosphorylation antibody (Cat. No.: AB-PN837)
Scientific Background: SCN3B (Sodium Voltage-Gated Channel Beta Subunit 3; Nav Beta 3)) is a gene that encodes a 24 kDa regulatory beta-subunit (Navb3) of voltage-gated sodium (Nav) channels. These channels are important for generating and propagating action potentials in excitable cells, such as neurons and cardiac muscle cells. SCN3B functions as a transmembrane glycoprotein that modulates the kinetics and density of the pore-forming alpha-subunit (most notably Nav1.5 in the heart). SCN3B belongs to the voltage-gated sodium channel group, and modulates channel gating kinetics (1). It features nn N-terminal signal sequence, an extracellular immunoglobulin-like domain, a single transmembrane region, and a C-terminal cytoplasmic region.SCN3B is known to form homodimers and homotrimers and associates non-covalently with alpha-subunits. It inactivates the sodium channel opening more slowly than its Beta1 subunit. It is also unique in causing persistent sodium currents that are thought to amplify summation of synaptic imputs which is likely to increase the excitability of specific neurons to their individual inputs (2). Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons (1, 2). SCN3B is expressed in the heart (particularly in ventricles and Purkinje fibers), the brain (high expression in the forebrain and hippocampus), and to a lesser extent in the kidneys and adrenal glands. It is found at the plasma membrane and in the Z disc of muscle cells. Defects in SCN3B are the cause of Brugada syndrome (3), atrial fibrillation, sudden infant death syndrome, cardiac conduction abnormality and neurodevelopmental disorders. This description may include information annotated by UniProt and/or Google AI.