Product Name:
Nav beta 3
Product Number:
ab-nn315-1
Target Full Name: Sodium channel subunit beta-3
Target Alias: Scn3b; KIAA1158
Product Type Specific: Sodium channel pan-specific antibody
Antibody Code: NN315-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9NY72
Protein SigNET: Q9NY72
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2B
Antibody Immunogen Source: Fusion protein amino acids 1-215 (full-length) of rat NavBeta3
Antibody Modification: Sodium channel pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~40 kDa protein by Western blotting.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: A 1:100 dilution of SMC-490 was sufficient for detection of NavBeta3 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-reat with NavBeta1, Navbeta2, or Navbeta4.
Related Product 1: Nav beta 2 pan-specific antibody (Cat. No.: AB-NN314-1)
Scientific Background: Nav Beta 3 (SCN3B) belongs to the voltage-gated sodium channel group, and modulates channel gating kinetics. It inactivates the sodium channel opening more slowly than its Beta1 subunit. It is also unique in causing persistent sodium currents which are thought to amplify summation of synaptic imputs which is likely to increase the excitability of specific neurons to their individual inputs (2). Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons (1, 2). Defects in SCN3B are the cause of Brugada syndrome (3).